Pulsed-field Gel Electrophoresis for Salmonella Infection
نویسندگان
چکیده
To detect Salmonella clusters, public health laboratories perform pulsed-field gel electrophoresis (PFGE) that provides a PFGE pattern, or DNA fingerprint. If the PFGE patterns of isolates from >2 persons are indistinguishable, the responsible bacteria may be related to a common source (1–3). PulseNet is a network of public health laboratories coordinated by the Centers for Disease Control and Prevention (CDC), in which bacteria that cause foodborne diseases, including Salmonella isolates, are analyzed by using PFGE. This network provides the means to rapidly compare PFGE patterns from isolates submitted in different geographic areas. State and local laboratories upload PFGE patterns to the national CDC PulseNet database. Indistinguishable patterns at the national level might represent a large multistate outbreak (4–6).
منابع مشابه
Subtyping of Salmonella enterica isolated from humans and food animals using Pulsed-Field Gel Electrophoresis
Salmonella infections are the second leading cause of zoonotic bacterial foodborne illness. Main source of infection in human is contaminated food products. The aim of this study was sub typing isolates of Salmonella entericaobtained during our previous study byPulsed Field Gel Electrophoresis (PFGE) technique. All 46 Salmonella isolates were serotyped and then subjected to PFGE. Total isolates...
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